February 8, 2018
Stephanie Shames to present Biochemistry and Molecular Biophysics Seminar today
Stephanie Shames, Division of Biology, will present "Elucidating the contribution of Legionella pneumophila effector proteins to pathogenesis and 1xbet online casino defense" as the first event in the Biochemistry and Molecular Biophysics Seminar series at 4 p.m. Thursday, Feb. 8, in 13 Leasure Hall.
Intracellular bacterial pathogens such as Legionella pneumophila cause disease by using specialized secretion systems to deliver bacterial effector proteins into a 1xbet online casino cell to manipulate biological pathways. A single strain of L. pneumophila encodes a repertoire of more than 300 different effector proteins, which are delivered into 1xbet online casino cells by the Dot/Icm type IV secretion system. Due to the large number of effector proteins, identifying their contribution to disease has proven to be challenging. We used insertion sequencing — INSeq — to reveal the contribution of effector genes to L. pneumophila virulence. The replication of hundreds of targeted effector mutants was measured in parallel in both a mouse model of Legionnaires’ disease, 1xbet online casino cells cultured ex vivo, and a natural amoeba 1xbet online casino . We uncovered phenotypes resulting from loss-of-function mutations in several effector genes in the mouse lung and in cultured 1xbet online casino cells. Phenotypes of two effectors identified in this screen, LegC4 and Lpg2505, were validated, which demonstrated the robustness of the screen. Our current work is geared toward understanding the role of these effectors in Legionella pathogenesis and 1xbet online casino defense.