Kansas State University

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Kansas State University
Department of Plant Pathology

4024 Throckmorton
1712 Claflin Road
Manhattan, KS 66506-5502
785-532-6176
785-532-5692 fax
wgrc@k-state.edu

Wheat Genetics Resource Center
Kansas Wheat Innovation Center

1990 Kimball Ave
Manhattan, KS 66502
785-320-4383

1xbet sports betting (ISH)

  1. Remove cover slips from the 1xbet sports betting that have been stored in a -70°C freezer with a razor blade; and dry the 1xbet sports betting in an ethanol series (75%, 95%, and 100% ethanol, 5 min each).
  2. Incubate 1xbet sports betting in hot phosphate buffer (1M NaH2PO4) at 92 ± 2°C for 2 min, rinse in tap water, and immediately stain with Giemsa solution.
  3. Briefly rinse 1xbet sports betting with tap water and dry with a hand puff.
  4. Record the 1xbet sports betting patterns of well-spread cells with a high-resolution objective that does not require a cover slip (i.e., Olympus NC Plan Apo 60 objective).
  5. Destain 1xbet sports betting in an ethanol series (75%, 95%, and 100% ethanol, 5 min each; 1xbet sports betting also can be briefly dipped several times in 45% acetic acid before the ethanol series to remove the Giemsa stain).