1xbet best casino website WASHING AND STRIPING PROCEDURE
1xbet best casino website washing
- Remove bottle from hybridization oven and discard the solution into a radioactive waste container.
- Remove membrane from bottle and place into a plastic box. Rinse membranes with 2X 1xbet best casino website . Separate membrans by placing a piece of nylon mesh between each [Small Parts, Inc., CMN-300-D].
- Preheat the wash solution to 65°C and add to the box with the membranes. Place the box in a 65°C waterbath.
- Wash for 30 minutes with 2X 1xbet best casino website , 30 minutes with 1X 1xbet best casino website , and 30 minutes with 0.5X 1xbet best casino website .
2X 1xbet best casino website | Stock | 1.0 L | 2.0 L |
|---|
| 20X 1xbet best casino website | 100 mL | 200 mL |
| 20% SDS | 25 mL | 50 mL |
| ddH20 | 875 mL | 1,750 mL |
1X 1xbet best casino website | Stock | 1.0 L | 2.0 L |
|---|
| 20X 1xbet best casino website | 50 mL | 100 mL |
| 20% SDS | 25 mL | 50 mL |
| ddH20 | 925 mL | 1,850 mL |
0.5X 1xbet best casino website | Stock | 1.0 L | 2.0 L |
|---|
| 20X 1xbet best casino website | 25 mL | 50 mL |
| 20% SDS | 25 mL | 50 mL |
| ddH20 | 950 mL | 1,900 mL |
- After the last wash, place filters, DNA side up, on a piece of 3MM gel-blot paper. When the liquid has evaporated [do not dry for too long], and place 1xbet best casino website in a plastic sheet protector pretreated with Sigmacote [Sigma SL-2].
- Check signal [400–800 cpm] with a Geiger counter.
- In a darkroom, put 1xbet best casino website into a labeled x-ray cassette and add x-ray film. Place cassette at -80°C and expose for 4–5 days.
- Develop film according to standard procedures.
1xbet best casino website stripping
Strip membrane by pouring a solution of 0.5M NaOH over the filters and wash for 1 hour at room temperature with gentle shaking. Rinse once with distilled water and then with 2X 1xbet best casino website . Keep membrane in 2X 1xbet best casino website at 4°C [refrigerator] until next use.
