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Kansas State University
Department of Plant Pathology

4024 Throckmorton
1712 Claflin Road
Manhattan, KS 66506-5502
785-532-6176
785-532-5692 fax
wgrc@k-state.edu

Wheat Genetics Resource Center
Kansas Wheat Innovation Center

1990 Kimball Ave
Manhattan, KS 66502
785-320-4383

1xbet online games login PROCEDURE

1xbet online games login

1xbet online games login buffer[for 4 probes]
ddH20
25.0 mL
20X SSPE
12.0 mL
1xbet sports betting Othe
2.0 mL
20% SDS
1.0 mL

1xbet sports bett[10 mg/mL; boil for 5 minutes and set on ice 5 minutes before adding]

0.2 mL
dextran sulfate
2.0 g
TOTAL
40.2 mL
  1. Replace prehybridization buffer in each 1xbet online games login bottle with 10 mL of 1xbet online games login buffer.
  2. Add probe to 1xbet online games login bottle.NOTE:do not allow the probe to come in direct contact with the membrane when first adding the probe. Make sure the probe goes directly into the 1xbet online games login solution in the bottle.
  3. Allow probes to hybridize for 18–24 hours at 65°C.