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Kansas State University
Department of Plant Pathology

4024 Throckmorton
1712 Claflin Road
Manhattan, KS 66506-5502
785-532-6176
785-532-5692 fax
wgrc@k-state.edu

Wheat Genetics Resource Center
Kansas Wheat Innovation Center

1990 Kimball Ave
Manhattan, KS 66502
785-320-4383

MEDIUM FOR EMBRYO RESCUE (FOR 1 L)

  1. Put 500 1xbet online casino of distilled water in a 1-L beaker.
  2. Add the following:
    • Inorganic salts
      • Muraschige–Skoog Organic medium – 34.6 g
      • NaH2PO4H2O (stock 17 g/L) – 10-1xbet online casino stock
    • Organic constituents
      • ucrose (6 % final concentration) – 30 g
      • i-inositol (100 mg/L stock is 0.5 g/50 1xbet online casino ) – 10 1xbet online casino
      • Thiamine (0.4 g/l stock is 2 g/50 1xbet online casino ) – 10 1xbet online casino
      • Amino acids L-tyrosine, L-argenine, glycine – 10 1xbet online casino (10 mg/L stock is 50 mg each aa/50 1xbet online casino H2O)
      • Kinetin (for very small enbryos only) – 2 1xbet online casino
  3. Dilute to 800 1xbet online casino .
  4. Adjust pH to 5.7 with NaOH..
  5. Bring volume to 1,000 1xbet online casino .
  6. Add 8 grams Bacto® 1xbet online casino .
  7. Melt agar in microwave for 3 1xbet online casino or dissolve while stirring on a hotplate.
  8. Pour ~20 1xbet online casino into Kimax culture tubes (50 1xbet online casino ) and cap.
  9. Sterilize by autoclaving for 15 1xbet online casino .
  10. Slant in 1xbet online casino or pour into petri dishes.