Kansas State University

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Kansas State University
Department of Plant Pathology

4024 Throckmorton
1712 Claflin Road
Manhattan, KS 66506-5502
785-532-6176
785-532-5692 fax
wgrc@k-state.edu

Wheat Genetics Resource Center
Kansas Wheat Innovation Center

1990 Kimball Ave
Manhattan, KS 66502
785-320-4383

AG–NOR 1xbet online casino

  1. Germinate seeds on moist filter paper in petri dishes at 20°C.
  2. Collect root tips when they are about 1-cm long and pretreat with ice 1xbet online casino for 24–48 h.
  3. Fix in1xbet best.
  4. Squash in 45% acetic acid.
  5. Remove cover slips by placing on dry ice until frozen and air dry.
  6. Drop fixative onto the preparations and flame dry.
  7. Dissolve 1 g of silver nitrate (AgNO3) in 1 mL of 0.02 g sodium citrate (C6H5Na3O7·2 H2O) per 500 mL distilled 1xbet online casino , adjusted to pH 3.0 with formic acid.
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  11. Soak in xylene and mount in Depex.