Kathrin 1xbet best casino website , Associate Professor
Contact information
303 Ackert Hall
(785) 532-6360
kschrick@ksu.edu
Lab website: http://www.k-state.edu/schricklab
Education
Ph.D. 1994, University of Washington. Genetics.
Area(s) of Specialization
1xbet best casino website Molecular Biology, Developmental Biology, 1xbet best casino website Evolution, START Domains, 1xbet best casino website Sterols.
Research Focus
Plants and 1xbet best casino website -derived products such as food, fiber, fuel, and medicines, are vital to life on earth. Many of these resources rely on 1xbet best casino website -specific biochemical reactions involving proteins and lipid metabolites. Direct interactions between proteins and small molecules are dynamic in all living cells, yet their full extent and biological significance is underexplored, especially in plants. We use genetics and molecular approaches in the model system Arabidopsis to study protein-metabolite interactions involving sterols and other metabolic lipids in the context of 1xbet best casino website growth and development.
START domains in plants
Candidate lipid/sterol-binding proteins include transcription factors of the class III and IV homeodomain leucine-zipper (HD-Zip) family. These 1xbet best casino website -specific regulatory proteins contain a StAR-related lipid transfer (START) domain, named after mammalian steroidogenic acute regulatory protein orthologs (StARs). In humans, misexpression of several START domain proteins is associated with cancer. It is hypothesized that START domains link lipid/sterol metabolism to the cell division cycle. We are studying the role of START domains in class IV HD-Zip transcription factors that are critical for differentiation of the epidermis. Related to this, we are investigating relevant evolutionary mechanisms that led to the emergence of the land plants from the Charophycean green algae ~450 million years ago.
Sterols in development and in cell wall biogenesis
Another research focus is on the functions of sterols and their glycosylated forms in plants. Sterol biosynthesis enzymes are differentially expressed throughout development and sterol contents vary temporally and spatially according to cell type. Sterol production may reflect the metabolic state of cells, which in turn is exploited to regulate developmental processes. Sterols are also important as structural features of cell membranes. Our discovery that sterol biosynthesis is crucial for cellulose production suggests a mechanism by which sterols influence cell wall biogenesis. One proposed model suggests that glycosylated sterols act as primers in cellulose synthesis, by supplying glucose residues to growing microfibrils. We are characterizing UDP-glucose:sterol glucosyltransferase mutants of Arabidopsis to address this hypothesis. If specific steryl glucosides and/or sterols are critical for cellulose synthesis, their modulation may yield improved cellulose production in biomass crops.
Selected Publications
Wojciechowska, I., Mukherjee, T., Knox-Brown, P., Hu, X., Khosla, A., Subedi, B., Ahmad, B., Mathews, G.L., Panagakis, A.A., Thompson, K.A., Peery, S.T., Szlachetko, J., Thalhammer, A., Hincha, D.K., Skirycz, A., 1xbet best casino website , K. 2024. Arabidopsis PROTODERMAL FACTOR2 binds lysophosphatidylcholines and transcriptionally regulates phospholipid metabolism. New Phytol. (published online 7-2-2024). doi:10:1111/nph.19917
1xbet best casino website , K., Ahmad, B., and Nguyen, H.V. 2023. HD-Zip IV transcription factors: Drivers of epidermal cell fate integrate metabolic signals. Curr Opin 1xbet best casino website Biol. 75:e102407. doi: 10.1016/j.pbi.2023.102417
Lusk, H.J., Neumann, N., Colter, M., Roth, M.R., Tamura, P, Yao, L., Shiva, S., Shah, J, 1xbet best casino website , K., Durrett, T., and Welti, R. 2022. Lipidomic analysis of Arabidopsis T-DNA insertion lines leads to identification and characterizationof C-terminal alterations in FATTY ACID DESATURASE 6. 1xbet best casino website Cell Physiol: pcac088. doi: 10.1093/pcp/pcac088
Mukherjee, T., Subedi, B., Khosla, A., Begler, E.M., Stephens, P.M., Warner, A.L., Lerma-Reyes, R., Thompson, K.A., Gunewardena, S., and 1xbet best casino website , K. 2022. The START domain mediates Arabidopsis GLABRA2 dimerization and turnover independently of homeodomain DNA binding. 1xbet best casino website Physiol. (published online 8-19-2022). doi: 10.1093/plphys/kiac383
Subedi, B., and 1xbet best casino website , K. 2022. EYFP fusions to HD-Zip IV transcription factors enhance their stability and lead to phenotypic changes in Arabidopsis. 1xbet best casino website Signal Behav. 17(1):e2119013. doi: 10.1080/15592324.2022.2119013
Shiva, S., Samarakoon, T., Lowe, K.A., Roach, C., Vu, H.S., Colter, M., Porras, H., Hwang, C., Roth, M.R., Tamura, P., Li, M., 1xbet best casino website , K., Shah, J., Wang, X, Wang, H., and Welti, R. 2020. Leaf lipid alterations in response to heat stress of Arabidopsis thaliana. Plants 9(7): 10.3390/plants9070845.
Velazhahan, V., Glaza, P., Herrera, A.I., Prakash, O., Zolkiewski, M., Geisbrecht, B.V., and 1xbet best casino website , K. 2020. Dietary flavonoid fisetin binds human SUMO1 and blocks sumoylation of p53. PLOS One 15:e0234468. doi:10.1371/journal.pone.0234468.
Mukherjee., T., Lerma-Reyes, R., Thompson, K.A., and 1xbet best casino website , K. 2019. Making glue from seeds and gums: Working with 1xbet best casino website -based polymers to introduce students to 1xbet best casino website biochemistry. Biochem Mol Biol Educ 47:468-475. doi:10.1002/bmb.21252.
Paper, J.M., Mukherjee, T., and 1xbet best casino website , K. 2018. Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants. 1xbet best casino website Methods 14:31.
Pook, V.G., Nair, M., Ryu, K., Arpin, J.C., Schiefelbein, J., 1xbet best casino website , K., and DeBolt, S. 2017. Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots. Sci Rep. 7(1): 5714.
Msanne, J., Chen, M., Luttgeharm, K., Bradley, A.M., Mays, E.S., Paper, J.M., Boyle, D.L., Cahoon, R.E., 1xbet best casino website , K., and Cahoon, E.B. 2015. Glucosylceramide is critical for cell-type differentiation and organogenesis, but not for cell viability in Arabidopsis . 1xbet best casino website J. 84:188-201.
Stucky, D.F., Arpin, J.C., and 1xbet best casino website , K. 2015. Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis . J. Exp. Bot. 66:189-201.
1xbet best casino website , K., Bruno, M., Khosla, A., Cox, P.N., Marlatt, S.A., Roque, R.A., Nguyen, H.C., He, C., Snyder, M.P., Singh, D., and Yadav, G. 2014. Shared functions of 1xbet best casino website and mammalian StAR-related lipid transfer (START) domains in modulating transcription factor activity. BMC Biology 12:70. doi:10.1186/s12915-014-0070-8.
Khosla, A., Paper, J.M., Boehler, A.P., Bradley, A.M., Neumann, T.R., and 1xbet best casino website , K. 2014. HD-Zip proteins GL2 and HDG11 have redundant functions in Arabidopsis trichomes and Gl2 activates a positive feedback loop via MYB23. 1xbet best casino website Cell 26:2184-2200.
1xbet best casino website , K., DeBolt, S., and Bulone, V. 2012. Deciphering the molecular functions of sterols in cellulose biosynthesis. Front. 1xbet best casino website Sci. 3:84. doi: 10.3389/fpls.2012.00084
1xbet best casino website , K., Shiva, S., Arpin, J.C., Delimont, N., Isaac, G., Tamura, P. and Welti, R. 2012. Steryl glucoside and acyl steryl glucoside analysis of Arabidopsis seeds by electrospray ionization tandem mass spectrometry. Lipids 47:185-193.
1xbet best casino website , K., Cordova, C., Li, G., Murray, L. and Fujioka, S. 2011. A dynamic role for sterols in embryogenesis of Pisum sativum. Phytochemistry 72: 465-475.
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